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日期:2022-11-30瀏覽:444次
植物鐵氧還蛋白-NADP氧化還原酶(FNR)活性引用文獻
【文獻標題】Positive effects of NaCl on the photoreaction and carbon assimilation efficiency in uaeda salsa
【作者】Qiang Li, Ru Liu, Zihan Li, Hai Fanet.al
【作者單位】山東師范大學 Sangdong Normal University
【文獻中引用產(chǎn)品】
植物鐵氧還蛋白-NADP氧化還原酶(FNR)活性
【關鍵詞】Abbreviations: Electron transport rate, ETR; Ferredoxin-NADP oxidoreductase, FNR; Ribulose-1 5-bisphosphate carboxylase, Rubisco; Rubisco activase, RCA.
【影響因子(IF)】4.2
【出版期刊】《Positive effects of NaCl on the photoreaction and carbon assimilation efficiency in uaeda salsa》
【產(chǎn)品原文引用】Determination of the activities of ferredoxin-NADP oxidoreductase, ribulose-1,5-bisphosphate arboxylase and Rubisco activase A total of 0.3 g of mature leaves were placed in a pre-cooled mortar, and the leaves were ground on ice with chilled 50 mM Tris buffer at pH 7.4. The supernatant was extracted by centrifugation and stored at -20 °C. The activity of FNR was assayed using a plant ELISA kit (Shanghai Huding Biotechnology Co., Ltd., Shanghai, China). A microplate reader (Spectra Max M5; Molecular Devices, San Jose, CA, USA) was used to assay the activity of the enzyme according to the manufacturer’s instructions (Zhao et al., 2018). Three replicates were established for each concentration of NaCl.The activities of Rubisco and RCA were assayed as described for FNR.
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