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日期:2020-11-30瀏覽:554次
隨著我國經(jīng)濟的發(fā)展、生活環(huán)境的變化、人們健康觀念的轉(zhuǎn)變以及老齡化進程的加快等因素影響,我國的生物醫(yī)藥產(chǎn)業(yè)也被看做朝陽產(chǎn)業(yè),發(fā)展勢頭迅猛。我國生物技術(shù)藥物的研究和開發(fā)起步較晚,目前,國產(chǎn)干擾素α的銷售*已經(jīng)超過了進口產(chǎn)品。我國shou創(chuàng)的一種新型重組人γ干擾素并已具備向國外轉(zhuǎn)讓技術(shù)和承包工程的能力,新一代干擾素正在研制之中。
今天小編給大家分享一篇山東師范大學的同學發(fā)表的一篇植物相關(guān)指標的文獻,IF2.3分。希望能對各位做科研的小伙伴們有幫助,也希望大家積極參與我們的fa論文贏獎學金的活動。
文獻展示:
【文獻標題】Transcriptome profiling of genes involved in photosynthesis in Elaeagnus angustifolia L. under salt stress
【作者】J. LIN, J.P. LI, F. YUAN,et.al
【作者單位】山東師范大學(Shandong Normal University)
【文獻中引用產(chǎn)品】
植物鎂螯合酶(MgCh) ELISA試劑盒
植物Rubisco活化酶(RCA)ELISA試劑盒
【關(guān)鍵詞】biomass; greening; ion concentrations; photosynthetic parameters; plant height.
【DOI】doi.org/10.1007/s11099-018-0824-6
【影響因子(IF)】2.3
【出版期刊】《PHOTOSYNTHETICA》
【產(chǎn)品原文引用】
Protein content and activity of magnesium chelatase and Rubisco:
Magnesium chelatase and Rubisco were prepared by first freezing a 0.2-g leaf sample in liquid nitrogen to prevent proteolytic activity, followed by grinding with 2 mL of extraction buffer (100 mM phosphate buffer, pH 7.2, containing 0.1 mM EDTA, and 2 mM ascorbic acid). The homogenate was centrifuged for 15 min at 10,000 rpm, and the supernatant was used to measure the protein content (ng L–1), magnesium chelatase activity [pmol(Mg-Deutero) min–1 mg–1(protein)] and Rubisco activity [μmol min–1 mg–1(protein)]. Magnesium chelatase and Rubisco were measured using a magnesium chelatase content kit and a Rubisco content kit (double antibody sandwich method, Huding Biological Technology Co., Shanghai, China) (3 replicates per treatment).
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