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日期:2023-03-15瀏覽:270次
上海滬鼎生物科技有限公司是國內(nèi)ELISA試劑盒優(yōu)質(zhì)供應商,代理銷售不同ELISA試劑盒品牌的進口/國產(chǎn)ELISA試劑盒,專業(yè)供應科研實驗所需的培養(yǎng)基,抗體,動物血清血漿,標準品對照品,化學試劑,酶聯(lián)免疫試劑盒,白介素試劑盒,金標檢測試劑盒,微生物,蛋白質(zhì),ELISA種屬涵蓋廣,憑借多年行業(yè)經(jīng)驗,完善的售后服務(wù),高質(zhì)量的產(chǎn)品。
滬鼎產(chǎn)品文獻:植物LOX,PLD,ELISA試劑盒引用文獻引用文獻
【文獻標題】High-pressure carbon dioxide treatment and vacuum packaging alleviate
the yellowing of peeled Chinese water chestnut (Eleocharis tuberosa)
【作者】Xuan Zhou,Xiaoyun Xu,Ayesha Murtaza,et.al
【作者單位】華中農(nóng)業(yè)大學(Huazhong Agricultural Universit)
【文獻中引用產(chǎn)品】
植物磷脂酶D(PLD)ELISA試劑盒
植物脂肪氧化酶(LOX)ELISA試劑盒 活性
【關(guān)鍵詞】Chinese Water Chestnut, Eleocharis tuberosa ,Yellowing ,High-pressure carbon dioxide, Vacuum packaging
【影響因子(IF)】8.74
【出版期刊】《Food Packaging and Shelf Life》
【產(chǎn)品原文引用】Phospholipase D (PLD) and lipid oxidase (LOX) activity. The
crude extracted enzyme solution was used to determine PLD and LOX
activities by following the instruction given in ELISA kit (Shanghai
Huding Biological Technology Co., Ltd.). The standard curve was
demonstrated using the standard concentration as the horizontal coordinate and the OD value as the vertical coordinate.
滬鼎產(chǎn)品文獻:斑馬魚卵黃原蛋白(VTG)ELISA試劑盒引用文獻
【文獻標題】Microcystin-LR influences the in vitro oocyte maturation of zebrafish by activating the MAPK pathway
【作者】Wanjing Liu, Chunhua Zhan, Tongzhou Zhang,et.al
【作者單位】華中農(nóng)業(yè)大學(Huazhong Agricultural University)
【文獻中引用產(chǎn)品】
斑馬魚卵黃原蛋白(VTG)ELISA試劑盒
【關(guān)鍵詞】Microcystin,Meiosis,Oocyte maturation,Hyper-phosphorylation,MAPK
【影響因子(IF)】7.2
【出版期刊】《Aquatic Toxicology》
【產(chǎn)品原文引用】
PP2A and MPF enzymatic activity and VTG content
PP2A enzymatic activity was assayed according to the instruction of Serine/Threonine Phosphatase Assay System (Promega, V2460;Madison, Wisconsin, USA). Live oocytes were rinsed and homogenized
in ice-cold PSB to obtain homogenate. The homogenate was added into the provided spin columns with Sephadex beads to remove endogenous phosphates. The supernatant was taken for detecting total soluble protein concentration using a bicinchoninic acid assay (BCA kit;Beyotime, Shanghai,China). The reaction was initiated by adding 15 μL of reaction premixes containing 5 μL of 1 mM phosphopeptide and 10 μL of PPase-2A 5×reaction buffer. After reaction at 37 °C for 30 min,50 μL of stop solution was added to each well. The data was read at 630 nm. The activity levels of MPF and the contents of VTG were determined through the zebrafish MPF ELISA Kit (Mlbio, Shanghai, China) and the zebrafish VTG ELISA Kit (Huding, Shanghai, China), following the manufacturer’sprotocol.
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