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日期:2023-04-06瀏覽:239次
今天小編給大家介紹一個(gè)老朋友——白細(xì)胞介素,它可是非常重要的,有著很強(qiáng)大的功能,由于最初是由白細(xì)胞產(chǎn)生又在白細(xì)胞間發(fā)揮作用,所以由此得名。
白細(xì)胞介素,簡稱白介素,是指在白細(xì)胞或免疫細(xì)胞間相互作用的淋巴因子,它和血細(xì)胞生長因子同屬細(xì)胞因子。兩者相互協(xié)調(diào),相互作用,共同完成造血和免疫調(diào)節(jié)功能。白細(xì)胞介素在傳遞信息,激活與調(diào)節(jié)免疫細(xì)胞,介導(dǎo)T、B細(xì)胞活化、增殖與分化及在炎癥反應(yīng)中起重要作用。
在對免疫應(yīng)答的研究過程中,在絲裂原刺激的細(xì)胞培養(yǎng)上清中發(fā)現(xiàn)了許多具有生物活性的分子,研究者各以自己測得的活性進(jìn)行命名,十幾年報(bào)道了近百種因子。后來借助分子生物學(xué)技術(shù)進(jìn)行比較研究發(fā)現(xiàn),以往許多以生物活性命名的因子實(shí)際上是具有多效性的同一物質(zhì)。為了避免命名的混亂,1979年第二屆國際淋巴因子專題會議將免疫應(yīng)答過程中白細(xì)胞間相互作用的細(xì)胞因子統(tǒng)一命名為白細(xì)胞介素(interleukin,il),在名稱后加阿拉伯?dāng)?shù)字編號以示區(qū)別,例如il-1、il-2……,新確定的因子依次命名。只有取行克隆化的基因、明確產(chǎn)物的性質(zhì)和活性才能得到國際會議的認(rèn)可。
白細(xì)胞介素是非常重要的細(xì)胞因子家族,它們在免疫細(xì)胞的成熟、活化、增殖和免疫調(diào)節(jié)等一系列過程中均發(fā)揮重要作用,此外它們還參與機(jī)體的多種生理及病理反應(yīng)。
滬鼎產(chǎn)品文獻(xiàn):人IL-6,TNF-α,iNOS ELISA試劑盒引用文獻(xiàn)
【文獻(xiàn)標(biāo)題】Rapeseed protein-derived ACE inhibitory peptides LY, RALP and GHS show
antioxidant and anti-inflammatory effects on spontaneously hypertensive rats
【作者】Rong He,Yujiao Wang,Yijie Yang,et.al
【作者單位】南京財(cái)經(jīng)大學(xué)(Nanjing University of Finance and Economics)
【文獻(xiàn)中引用產(chǎn)品】
人白細(xì)胞介素6(IL-6)ELISA試劑盒
人腫瘤壞死因子α(TNF-α)ELISA試劑盒
人誘導(dǎo)型一氧化氮合成酶(iNOS)ELISA試劑盒
【關(guān)鍵詞】Rapeseed peptide,ACE inhibition,Anti-inflammatory effect,Cytokine,Oxidative stress
【影響因子(IF)】5.9
【出版期刊】《Journal of Functional Foods》
【產(chǎn)品原文引用】
Materials and reagents
RAW264.7 cells were obtained from the cell bank of the Chinese Academy of Sciences(Shanghai,China). LPS from Escherichia coli O55:B5,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT), fetal bovine serum, and hematoxylin and eosin (H&E) solutions were purchased from Sigma-Aldrich Trading Co., Ltd. (Shanghai,China). The SOD Assay Kit, Malondialdehyde (MDA) Assay Kit, Nitric Oxide (NO) Assay Kit and Nitric Oxide Synthase (NOS) Assay Kit were purchased from Jiancheng Bioengineering Institute (Nanjing, China).The Human Interleukin 6 (IL-6) ELISA Kit, Tumor Necrosis Factor-α(TNF-α) ELISA Kit and Inducible Nitric Oxide Synthase (iNOS) Assay Kit were purchased from Shanghai HuDing Biological Technology Co., Ltd.(Shanghai, China). Phosphate-buffered saline (PBS, pH 7.4) was purchased from Solarbio Science and Technology Co., Ltd. (Beijing, China).All other chemical reagents were of analytical grade. The ACE inhibitory peptides Leu-Tyr (LY), Arg-Ala-Leu-Pro (RALP) and Gly-His-Ser(GHS) were synthesized with purity over 95% by Synthgene Biological Technology Co., Ltd. (Shanghai, China).